指标别名
betacellulin; BTC; Probetacellulin
免疫原
Expression system for standard: E.coli; Immunogen sequence: D32-Y111
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse BTC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1193)
背景资料
Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1193-CAP | 96-well plate precoated with anti- mouse BTC antibody | 1 |
EK1193-ST | Lyophilized recombinant mouse BTC standard | 10ng/tube×2 |
EK1193-DA | Biotinylated anti- mouse BTC antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 11 | 59 | 318 | 10 | 63 | 326 |
Standard deviation | 0.79 | 4.66 | 22.26 | 0.76 | 5.04 | 28.68 |
CV(%) | 7.2% | 7.9% | 7% | 7.6% | 8% | 8.8% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.100 | 0.118 | 0.128 | 0.171 | 0.253 | 0.421 | 0.837 | 1.801 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)