Mouse AFP ELISA Kit

96T/2480

AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP

Expression system for standard: NS0; Immunogen sequence: K19-V605

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

312pg/ml-20000pg/ml

Afp

alpha fetoprotein

P02772

Mouse AFP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1661)

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Catalog number	Description	Quantity
EK1661-CAP	96-well plate precoated with anti- mouse AFP antibody	1
EK1661-ST	Lyophilized recombinant mouse AFP standard	10ng/tube×2
EK1661-DA	Biotinylated anti- mouse AFP antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	50	189	834	57	204	928
Standard deviation	2.40	9.26	41.70	2.91	12.04	59.39
CV(%)	4.8%	4.9%	5.0%	5.1%	5.9%	6.4%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.006	0.065	0.134	0.269	0.466	0.924	1.552	2.366

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h