Human BMP10 ELISA Kit

96T/2800

BMP 10; BMP10; BMP-10; bone morphogenetic protein 10

NSO, N314-R421

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

15.6 pg/ml - 1000 pg/ml

BMP10

bone morphogenetic protein 10

O95393  

Human BMP10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1808)

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 2p13.3. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.

15-20分钟

Catalog number	Description	Quantity
EK1808-CAP	96-well plate precoated with anti- human BMP-10 antibody	1
EK1808-ST	Lyophilized recombinant human BMP-10 standard	10ng/tube×2
EK1808-DA	Biotinylated anti- human BMP-10 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	40	207	429	52	231	462
Standard deviation	2.08	11.8	19.8	15.71	16.63	35.57
CV(%)	5.2	5.7	4.6	6.8	7.2	7.7

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.003	0.085	0.158	0.328	0.591	1.152	1.943	2.722

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate )

3.5h