Rat BMP10 ELISA Kit

96T/2800

BMP 10; BMP10; BMP-10; bone morphogenetic protein 10

Expression system for standard: NS0; Immunogen sequence: N314-R421

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

15.6pg/ml-1000pg/ml

Bmp10

bone morphogenetic protein 10

Q4AEG6  

Rat BMP10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1810)

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 4q34. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.

15-20分钟

Catalog number	Description	Quantity
EK1810-CAP	96-well plate precoated with anti- rat BMP-10 antibody	1
EK1810-ST	Lyophilized recombinant rat BMP-10 standard	10ng/tube×2
EK1810-DA	Biotinylated anti- rat BMP-10 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	34	244	404	37	249	456
Standard deviation	1.87	12.4	17.38	2.26	16.4	34.2
CV(%)	5.5%	5.1%	4.3%	6.1%	6.6%	7.5%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.003	0.085	0.158	0.328	0.591	1.152	1.943	2.722

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)