Mouse BMP10 ELISA Kit

96T/2800

BMP 10; BMP10; BMP-10; bone morphogenetic protein 10

Expression system for standard: NS0; Immunogen sequence: N314-R421

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

15.6pg/ml-1000pg/ml

Bmp10

bone morphogenetic protein 10

Q9R229  

Mouse BMP10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1809)

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 6; 6 D1. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.

Catalog number	Description	Quantity
EK1809-CAP	96-well plate precoated with anti- human mouse BMP antibody	1
EK1809-ST	Lyophilized recombinant human mouse BMP standard	10ng/tube×2
EK1809-DA	Biotinylated anti- human mouse BMP antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	29	211	444	31	226	487
Standard deviation	1.19	9.5	27.53	1.71	13.1	37.01
CV(%)	4.1%	4.5%	6.2%	5.5%	5.8%	7.6%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.003	0.085	0.158	0.328	0.591	1.152	1.943	2.722

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h