Anti-Transferrin/TF Antibody (Clone#OTI5G2)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF FCM

M00094-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-Transferrin/TF antibody (M00094-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Transferrin/TF antigen affinity purified monoclonal antibody (M00094-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Transferrin/TF at approximately 77 kDa. The expected band size for Transferrin/TF is at 77 kDa.
all(3)
Anti-TF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF .
all(3)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody, and then analyzed by flow cytometry.
all(3)

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产品名称

Anti-Transferrin/TF Antibody (Clone#OTI5G2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Beta 1 metal binding globulin; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF, FCM

基因名称

克隆号

OTI5G2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human TF(NP_001054) produced in HEK293T cell.

计算分子量

77 kDa

实际分子量

77 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1%(4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover(25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of transferrin for Fe(III) is extremely high(1023 M 1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.

Uniprot ID

P02787

文献引用格式

Transferrin/TF Antibody (Clone#OTI5G2) (Boster Biological Technology, Wuhan, China. Catalog#M00094-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transferrin

蛋白名全称

Serotransferrin(Transferrin)

实验方案>

实验步骤

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