Western blot (WB): | 1:200-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |
Figure 1. Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).
Figure 2. Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TF (Cat# M00094-4)(1:2000).
Figure 4. Anti-TF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF .
Figure 5. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody, and then analyzed by flow cytometry.
Figure 1. Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).
Figure 2. Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TF (Cat# M00094-4)(1:2000).
Figure 4. Anti-TF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF .
Figure 5. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody, and then analyzed by flow cytometry.