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Anti-Transferrin/TF Antibody (Clone#OTI5G2)

Mouse monoclonal antibody

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筛选器: All WB ICC/IF FCM

M00094-4

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).

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  • Figure 2. Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).

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  • Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TF (Cat# M00094-4)(1:2000).

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  • Figure 4. Anti-TF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF .

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  • Figure 5. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody, and then analyzed by flow cytometry.

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产品名称
Anti-Transferrin/TF Antibody (Clone#OTI5G2)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Beta 1 metal binding globulin; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin
产品类型
Monoclonal
检验物种
human
应用范围
WB,ICC/IF,FCM
基因名称
TF
克隆号
OTI5G2
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human TF(NP_001054) produced in HEK293T cell.
计算分子量
77 kDa
实际分子量
77 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1%(4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover(25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of transferrin for Fe(III) is extremely high(1023 M 1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.
Uniprot ID
P02787  
文献引用格式
Transferrin/TF Antibody (Clone#OTI5G2) (Boster Biological Technology, Wuhan, China. Catalog#M00094-4)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
transferrin
蛋白名全称
Serotransferrin(Transferrin)
推荐稀释比
Western blot (WB):1:200-2000
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow cytometry (FCM):1:100

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    Anti-Transferrin/TF Antibody (Clone#OTI5G2)

    筛选器: All WB ICC/IF FCM

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