Anti-Transferrin/TF Antibody

筛选器： All WB IHC FCM ELISA

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  Western blot analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
  Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat RH-35 whole cell lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse Hepa1/6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Transferrin/TF antigen affinity purified polyclonal antibody (RP1022) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Transferrin/TF at approximately 77 kDa. The expected band size for Transferrin/TF is at 77 kDa.

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  IHC analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022) .
  Transferrin/TF was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-Transferrin/TF Antibody (RP1022) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of HepG2 cells using anti-Transferrin/TF antibody (RP1022).
  Overlay histogram showing HepG2 cells stained with RP1022 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Transferrin/TF Antibody (RP1022) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
  Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat RH-35 whole cell lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse Hepa1/6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Transferrin/TF antigen affinity purified polyclonal antibody (RP1022) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Transferrin/TF at approximately 77 kDa. The expected band size for Transferrin/TF is at 77 kDa.

• 

  IHC analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022) .
  Transferrin/TF was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-Transferrin/TF Antibody (RP1022) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of HepG2 cells using anti-Transferrin/TF antibody (RP1022).
  Overlay histogram showing HepG2 cells stained with RP1022 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Transferrin/TF Antibody (RP1022) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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