Anti-Transferrin/TF Antibody (Clone#7I11B10)

Mouse monoclonal antibody

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筛选器： All WB

M00094-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Transferrin/TF using anti-Transferrin/TF antibody (M00094-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Transferrin/TF antigen affinity purified monoclonal antibody (M00094-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Transferrin/TF at approximately 77 kDa. The expected band size for Transferrin/TF is at 77 kDa.
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产品名称

Anti-Transferrin/TF Antibody (Clone#7I11B10)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Beta 1 metal binding globulin; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

克隆号

7I11B10

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Transferrin, different from the related mouse and rat sequences by five amino acids.

计算分子量

77 kDa

实际分子量

77 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. Transferrin consists of a polypeptide chain containing 679 amino acids in humans. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). And Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of transferrin for Fe(III) is extremely high (1023 M?1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.

Uniprot ID

P02787

文献引用格式

Transferrin/TF Antibody (Clone#7I11B10) (Boster Biological Technology, Wuhan, China. Catalog#M00094-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transferrin

蛋白名全称

Serotransferrin

实验方案>

实验步骤

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