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Anti-Ki67/MKI67 Antibody (Clone#5E12)

mouse monoclonal antibody

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筛选器: All IHC IF ICC/IF FCM

M00254-8

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. IHC analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(8)
  • Figure 2. IHC analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(8)
  • Figure 3. IHC analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(8)
  • Figure 4. IHC analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(8)
  • Figure 5. IHC analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(8)
  • Figure 6. IF analysis using anti- Ki67 antibody (M00254-8). detected in paraffin-embedded section of human tonsil cancer tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).

    all(8)
  • Figure 7. ICC analysis using anti- Ki67 antibody (M00254-8). was detected in immersion fixed A549 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).

    all(8)
  • Figure 8. Flow cytometry analysis of Jurkat cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

    all(8)

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产品名称
Anti-Ki67/MKI67 Antibody (Clone#5E12)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
D630048A14Rik; Ki 67; Ki67; Mki67
产品类型
Monoclonal
检验物种
human
应用范围
IHC, IF, ICC/IF, FCM
基因名称
MKI67
克隆号
5E12
宿主
Mouse
抗体亚型
IgG2b
免疫原
E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
protein G purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Uniprot ID
P46013  
RRID
文献引用格式
Ki67/MKI67 Antibody (Clone#5E12) (Boster Biological Technology, Wuhan, China. Catalog#M00254-8)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
antigen identified by monoclonal antibody Ki-67
蛋白名全称
Antigen KI-67
推荐稀释比
Immunohistochemistry (IHC): 1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Immunofluorescence (IF):1:50-400
Flow Cytometry (Fixed):1:50-200
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Ki67/MKI67 Antibody (Clone#5E12)

    筛选器: All IHC IF ICC/IF FCM

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