Figure 1. Western blot analysis of Ki67 using anti-Ki67 antibody (M00254-9).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates.Use mouse anti- Ki67 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Ki67 at approximately 358 kDa. The expected band size for Ki67 is at 358 kDa.
all(8)
Figure 2. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(8) 
Figure 3. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human lymphomas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(8) 
Figure 4. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(8) 
Figure 5. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(8) 
Figure 6. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(8) 
Figure 7. ICC analysis using anti- Ki67 antibody (M00254-9).was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(8) 
Figure 8. Flow cytometry analysis of Jurkat cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(8) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Ki67 using anti-Ki67 antibody (M00254-9).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates.Use mouse anti- Ki67 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Ki67 at approximately 358 kDa. The expected band size for Ki67 is at 358 kDa.
Figure 2. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human lymphomas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 7. ICC analysis using anti- Ki67 antibody (M00254-9).was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 8. Flow cytometry analysis of Jurkat cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of Ki67 using anti-Ki67 antibody (M00254-9).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates.Use mouse anti- Ki67 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Ki67 at approximately 358 kDa. The expected band size for Ki67 is at 358 kDa.
Figure 2. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human lymphomas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6. IHC analysis using anti- Ki67 antibody (M00254-9). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 7. ICC analysis using anti- Ki67 antibody (M00254-9).was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 8. Flow cytometry analysis of Jurkat cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
