Figure 1. Western blot analysis of anti- CD45/PTPRC Antibody (M00555-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
Use mouse anti-CD45/PTPRC 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD45/PTPRC at approximately 147KD. The expected band size for CD45/PTPRC is at 147KD.
Figure 2. IF analysis using anti- CD45/PTPRC Antibody (M00555-6). detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(4) 
Figure 3. Flow cytometry analysis of Raji cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(4) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- CD45/PTPRC Antibody (M00555-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
Use mouse anti-CD45/PTPRC 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD45/PTPRC at approximately 147KD. The expected band size for CD45/PTPRC is at 147KD.
Figure 2. IF analysis using anti- CD45/PTPRC Antibody (M00555-6). detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of Raji cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- CD45/PTPRC Antibody (M00555-6). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
Use mouse anti-CD45/PTPRC 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD45/PTPRC at approximately 147KD. The expected band size for CD45/PTPRC is at 147KD.
Figure 2. IF analysis using anti- CD45/PTPRC Antibody (M00555-6). detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of Raji cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
