Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse ovary tissue lysate. -FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for FGF2 at approximately 18KD. The expected band size for FGF2 is at 17KD.
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse ovary tissue lysate. -FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for FGF2 at approximately 18KD. The expected band size for FGF2 is at 17KD.