Anti-FGF2 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of anti-FGF2 antibody (BA14189). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human SK-OV-3 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: human U87 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (BA14189) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.

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  Figure 2. IF analysis of FGF2 using anti-FGF2 antibody (BA14189).
  FGF2 was detected in an immunocytochemical section of SiHa cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 3. Flow Cytometry analysis of SiHa cells using anti-FGF2 antibody (BA14189).
  Overlay histogram showing SiHa cells stained with BA14189 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGF2 Antibody (BA14189, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Figure 1. Western blot analysis of anti-FGF2 antibody (BA14189). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human SK-OV-3 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: human U87 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (BA14189) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.

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  Figure 2. IF analysis of FGF2 using anti-FGF2 antibody (BA14189).
  FGF2 was detected in an immunocytochemical section of SiHa cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 3. Flow Cytometry analysis of SiHa cells using anti-FGF2 antibody (BA14189).
  Overlay histogram showing SiHa cells stained with BA14189 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGF2 Antibody (BA14189, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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