Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (A00121-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
Figure 2. IHC analysis of FGF2 using anti-FGF2 antibody (A00121-1) .
FGF2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-FGF2 Antibody (A00121-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IF analysis of FGF2 using anti-FGF2 antibody (A00121-1).
FGF2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-FGF2 Antibody (A00121-1) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ELISA(Cap): | 1:50-1:200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (A00121-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
Figure 2. IHC analysis of FGF2 using anti-FGF2 antibody (A00121-1) .
FGF2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-FGF2 Antibody (A00121-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IF analysis of FGF2 using anti-FGF2 antibody (A00121-1).
FGF2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-FGF2 Antibody (A00121-1) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody.
Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (A00121-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
Figure 2. IHC analysis of FGF2 using anti-FGF2 antibody (A00121-1) .
FGF2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-FGF2 Antibody (A00121-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IF analysis of FGF2 using anti-FGF2 antibody (A00121-1).
FGF2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-FGF2 Antibody (A00121-1) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody.
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