Figure 1. Western blot analysis of anti- FGF2 Antibody (A00121-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: SK-OV-3 whole cell lysates,
Lane 4: Hela whole cell lysates.
Use rabbit anti- FGF2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FGF2 at approximately 18KD. The expected band size for FGF2 is at 31KD.
Figure 2. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of human gastric cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
all(5) 
Figure 3. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of mouse cerebellum tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
all(5) 
Figure 4. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of rat cerebellum tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
all(5) 
Figure 5. ICC analysis using anti- FGF2 Antibody (A00121-3). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
all(5) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- FGF2 Antibody (A00121-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: SK-OV-3 whole cell lysates,
Lane 4: Hela whole cell lysates.
Use rabbit anti- FGF2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FGF2 at approximately 18KD. The expected band size for FGF2 is at 31KD.
Figure 2. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of human gastric cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 3. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of mouse cerebellum tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 4. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of rat cerebellum tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 5. ICC analysis using anti- FGF2 Antibody (A00121-3). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of anti- FGF2 Antibody (A00121-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: SK-OV-3 whole cell lysates,
Lane 4: Hela whole cell lysates.
Use rabbit anti- FGF2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FGF2 at approximately 18KD. The expected band size for FGF2 is at 31KD.
Figure 2. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of human gastric cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 3. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of mouse cerebellum tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 4. IHC analysis using anti- FGF2 Antibody (A00121-3). detected in paraffin-embedded section of rat cerebellum tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 5. ICC analysis using anti- FGF2 Antibody (A00121-3). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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