Figure 1. Western blot analysis of Bax using anti-Bax antibody (A00183). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: HEPA1-6 whole cell lysates,Lane 4: HELA whole cell lysates,Lane 5: MCF-7 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bax at approximately 21KD. The expected band size for Bax is at 21KD.
all(6)
Figure 2. IHC analysis of Bax using anti-Bax antibody (A00183).detected in paraffin-embedded section of mouse intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 3. IHC analysis of Bax using anti-Bax antibody (A00183). detected in paraffin-embedded section of rat intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 4. IHC analysis of Bax using anti-Bax antibody (A00183).detected in paraffin-embedded section of human intetsinal cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183).s detected in paraffin-embedded section of human lung cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 6. Flow Cytometry analysis of A549 cells using anti-Bax antibody (A00183).Overlay histogram showing A549 cells stained with A00183 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(6) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Bax using anti-Bax antibody (A00183). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: HEPA1-6 whole cell lysates,Lane 4: HELA whole cell lysates,Lane 5: MCF-7 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bax at approximately 21KD. The expected band size for Bax is at 21KD.
Figure 2. IHC analysis of Bax using anti-Bax antibody (A00183).detected in paraffin-embedded section of mouse intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Bax using anti-Bax antibody (A00183). detected in paraffin-embedded section of rat intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Bax using anti-Bax antibody (A00183).detected in paraffin-embedded section of human intetsinal cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183).s detected in paraffin-embedded section of human lung cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. Flow Cytometry analysis of A549 cells using anti-Bax antibody (A00183).Overlay histogram showing A549 cells stained with A00183 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of Bax using anti-Bax antibody (A00183). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: HEPA1-6 whole cell lysates,Lane 4: HELA whole cell lysates,Lane 5: MCF-7 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bax at approximately 21KD. The expected band size for Bax is at 21KD.
Figure 2. IHC analysis of Bax using anti-Bax antibody (A00183).detected in paraffin-embedded section of mouse intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Bax using anti-Bax antibody (A00183). detected in paraffin-embedded section of rat intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Bax using anti-Bax antibody (A00183).detected in paraffin-embedded section of human intetsinal cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183).s detected in paraffin-embedded section of human lung cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. Flow Cytometry analysis of A549 cells using anti-Bax antibody (A00183).Overlay histogram showing A549 cells stained with A00183 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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