Anti-BAX Antibody

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC IP FCM

BM3964

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-BAX antibody (BM3964). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAX antigen affinity purified monoclonal antibody (BM3964) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAX at approximately 21 kDa. The expected band size for BAX is at 21 kDa.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Bax Antibody.
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产品简介>

产品名称

Anti-BAX Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

Apoptosis regulator BAX; BAX

产品类型

Monoclonal

检验物种

human, mouse, rat, hamster

应用范围

WB, IHC, IP, FCM

基因名称

BAX

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Bax

计算分子量

21 kDa

实际分子量

21 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Uniprot ID

Q07812

文献引用格式

BAX Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM3964)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Apoptosis regulator BAX

实验方案>

实验步骤

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