Anti-BAX Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of Bax using anti- Bax antibody (BA0315-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Rat Testis Tissue Lysate
  Lane 2: Rat Kidney Tissue Lysate
  Lane 3: Rat Brain Tissue Lysate
  Lane 4: Rat Ovary Tissue Lysate
  Lane 5: HELA Cell Lysate
  Lane 6: MM231 Cell Lysate
  Lane 7: A549 Cell Lysate
  Lane 8: JURKAT Cell Lysate
  Lane 9: Human Placenta Tissue Lysate
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Bax antigen affinity purified polyclonal antibody (Catalog # BA0315-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.

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  Figure 2. IHC analysis of BAX using anti-BAX antibody (BA0315-2).
  BAX was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. Flow Cytometry analysis of A431 cells using anti-BAX antibody (BA0315-2).Overlay histogram showing A431 cells stained with BA0315-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAX Antibody (BA0315-2, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.