Anti-BAX Antibody (Clone#OTI2A1)

筛选器： All WB IHC

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  Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and BAX-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BAX monoclonal antibody M00183-4. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control (1:500).

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  Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BAX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M00183-4) (1:2000)

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  Figure 3. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BAX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M00183-4) (1:2000)

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  Figure 4. Western blot analysis of extracts (35ug) from 6 different cell lines by using anti-BAX monoclonal antibody (1:500).

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  Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAX (1:2000) (Cat# M00183-4).