Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of NGF/NGF Beta using anti-NGF/NGF Beta antibody (A00341). Lane 1: Recombinant Human NGF/NGF Beta Protein 0.5ng -NGF/NGF Beta antigen affinity purified polyclonal antibody (Catalog # A00341) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NGF/NGF Beta at approximately 13KD. The expected band size for NGF/NGF Beta is at 13KD
Figure 1. Western blot analysis of NGF/NGF Beta using anti-NGF/NGF Beta antibody (A00341). Lane 1: Recombinant Human NGF/NGF Beta Protein 0.5ng -NGF/NGF Beta antigen affinity purified polyclonal antibody (Catalog # A00341) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NGF/NGF Beta at approximately 13KD. The expected band size for NGF/NGF Beta is at 13KD