Anti-NGF beta/NGF Antibody

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4100

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-NGF beta/NGF antibody (BM4100). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NGF beta/NGF antigen affinity purified monoclonal antibody (BM4100) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NGF beta/NGF at approximately 35 kDa. The expected band size for NGF beta/NGF is at 27 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using NGF Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of SH-SY5Y cells, using NGF Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NGF beta/NGF Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

NGF; NGFB; Beta-nerve growth factor; Beta-NGF

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

NGF

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NGF

计算分子量

27 kDa

实际分子量

35 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. It is mapped to 1p13.2. This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Uniprot ID

P01138

文献引用格式

NGF beta/NGF Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4100)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Beta-nerve growth factor

实验方案>

实验步骤

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