Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- NGF Antibody (A00341-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U-87MG whole cell lysates,
Lane 2: PC-3 whole cell lysates.
Use rabbit anti- NGF/NGF β 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NGF/NGF β at approximately 27KD. The expected band size for NGF/NGF β is at 27KD.
Figure 1. Western blot analysis of anti- NGF Antibody (A00341-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U-87MG whole cell lysates,
Lane 2: PC-3 whole cell lysates.
Use rabbit anti- NGF/NGF β 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NGF/NGF β at approximately 27KD. The expected band size for NGF/NGF β is at 27KD.