Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti-NGF antibody (A00341-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates,Lane 2: human 22RV1 whole cell lysates,Lane 3: human A549 whole cell lysates,Use rabbit anti- NGF 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NGF at approximately 27-35KD. The expected band size for NGF is at 27KD.
Figure 1. Western blot analysis of anti-NGF antibody (A00341-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates,Lane 2: human 22RV1 whole cell lysates,Lane 3: human A549 whole cell lysates,Use rabbit anti- NGF 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NGF at approximately 27-35KD. The expected band size for NGF is at 27KD.