Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
ELISA: | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-3). Lane 1: rat kidney tissue lysates. anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
Figure 2. IHC analysis of IL12B using anti-IL12B antibody (A01152-3).IL12B was detected in paraffin-embedded section of rat spleen tissue. anti-IL12B Antibody (A01152-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-3). Lane 1: rat kidney tissue lysates. anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
Figure 2. IHC analysis of IL12B using anti-IL12B antibody (A01152-3).IL12B was detected in paraffin-embedded section of rat spleen tissue. anti-IL12B Antibody (A01152-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.