Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-4). Lane 1: mouse NIH3T3 cell lysates, Lane 2: human A549 cell lysates. anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-4)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.
Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-4). Lane 1: mouse NIH3T3 cell lysates, Lane 2: human A549 cell lysates. anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-4)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.