Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of Anti-IL12B antibody (RP1028). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Recombinant rat IL12 P40 protein,Use rabbit Anti-IL12B 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).
Figure 1. Western blot analysis of Anti-IL12B antibody (RP1028). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Recombinant rat IL12 P40 protein,Use rabbit Anti-IL12B 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).