Anti-PCNA Antibody

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3888

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- PCNA antibody (BM3888). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: U-937 whole cell lysates,
Lane 4: 293T whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: rat RAW264.7 tissue lysates.
Use mouse anti- PCNA 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.
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Figure 2. IHC analysis using anti- PCNA antibody (BM3888). detected in paraffin-embedded section of paraffin-embedded human colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of PCNA using anti-PCNA antibody (BM3888) and anti-Beta Tubulin antibody (M01857-3).
PCNA was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-PCNA Antibody (BM3888) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133) were used as secondary antibody.
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产品简介>

产品名称

Anti-PCNA Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

PCNA

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

PCNA

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PCNA

计算分子量

29 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Uniprot ID

P12004

RRID

AB_2861422

文献引用格式

PCNA Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM3888)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Proliferating cell nuclear antigen

实验方案>

实验步骤

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