Anti-PCNA Antibody (Clone#OTI4G3)

Mouse monoclonal antibody

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筛选器： All IHC WB

M00125-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)
all(13)
Western blot analysis of extracts (15ug) from 2 different cell lines by using anti-PCNA monoclonal antibody (293T, lefe lane; Hela, right lane). Dilution: 1:2000
all(13)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
all(13)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PCNA Antibody (Clone#OTI4G3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

PCNA

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

PCNA

克隆号

OTI4G3

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.

计算分子量

29 kDa

实际分子量

28.6 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Uniprot ID

P12004

文献引用格式

PCNA Antibody (Clone#OTI4G3) (Boster Biological Technology, Wuhan, China. Catalog#M00125-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

proliferating cell nuclear antigen

蛋白名全称

Proliferating cell nuclear antigen

实验方案>

实验步骤

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