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Anti-PCNA Antibody (Clone#OTI4G3)

Mouse monoclonal antibody

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筛选器: All IHC WB

M00125-4

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 2. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 4. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 6. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 8. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 9. Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 10. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00125-4)

    all(13)
  • Figure 12. Western blot analysis of extracts (15ug) from 2 different cell lines by using anti-PCNA monoclonal antibody (293T, lefe lane; Hela, right lane). Dilution: 1:2000

    all(13)
  • Figure 13. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.

    all(13)

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产品简介>

产品名称
Anti-PCNA Antibody (Clone#OTI4G3)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
PCNA
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
PCNA
克隆号
OTI4G3
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.
计算分子量
29 kDa
实际分子量
28.6 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Uniprot ID
P12004  
文献引用格式
PCNA Antibody (Clone#OTI4G3) (Boster Biological Technology, Wuhan, China. Catalog#M00125-4)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
proliferating cell nuclear antigen
蛋白名全称
Proliferating cell nuclear antigen
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150

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    Anti-PCNA Antibody (Clone#OTI4G3)

    筛选器: All IHC WB

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