Figure 1. Western blot analysis of anti- PCNA Antibody (M00125-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hek293 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Colo320 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: Rwa264.7 whole cell lysates.
Use mouse anti- PCNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.
Figure 2. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human clymphadenoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 3. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 4. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 5. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 6. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 7. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 8. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of Rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(10) 
Figure 9. ICC analysis using anti- PCNA Antibody (M00125-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(10) 
Figure 10. Flow cytometry analysis of JK cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(10) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- PCNA Antibody (M00125-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hek293 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Colo320 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: Rwa264.7 whole cell lysates.
Use mouse anti- PCNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.
Figure 2. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human clymphadenoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 7. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 8. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of Rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 9. ICC analysis using anti- PCNA Antibody (M00125-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 10. Flow cytometry analysis of JK cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- PCNA Antibody (M00125-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hek293 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Colo320 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: Rwa264.7 whole cell lysates.
Use mouse anti- PCNA 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.
Figure 2. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human clymphadenoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 7. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 8. IHC analysis using PCNA Antibody (M00125-3). detected in paraffin-embedded section of Rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 9. ICC analysis using anti- PCNA Antibody (M00125-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 10. Flow cytometry analysis of JK cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
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