Anti-ACLY Antibody

Rabbit monoclonal antibody

说明书

筛选器： All WB IP IHC ICC/IF FCM

BM4399

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期： 5-7天

加入购物车立即购买

Figure 1. Western blot analysis of anti-ACLY antibody (BM4399). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACLY antigen affinity purified monoclonal antibody (BM4399) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACLY at approximately 121 kDa. The expected band size for ACLY is at 121 kDa.
all(5)
Immunoprecipitate (IP) analysis using the Antibody. (wb)
all(5)
Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody .
all(5)
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ACLY Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IP, IHC, ICC/IF, FCM

基因名称

ACLY

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ATP citrate lyase

计算分子量

121 kDa

实际分子量

121 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Uniprot ID

P53396

文献引用格式

ACLY Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4399)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

ATP-citrate synthase

实验方案>

实验步骤

引用文献(0)>

清除