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Anti-ACLY Antibody (Clone#OTI3G8)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF

M02372-2

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ACLY monoclonal antibody (1:200).

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  • Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACLY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACLY(Cat# M02372-2).

    all(19)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 4. Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 5. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 8. Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 9. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 10. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 12. Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 13. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 14. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 15. Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 16. Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 17. Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 18. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

    all(19)
  • Figure 19. Anti-ACLY mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY .

    all(19)

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产品简介>

产品名称
Anti-ACLY Antibody (Clone#OTI3G8)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP
产品类型
Monoclonal
检验物种
human,mouse,rat
应用范围
WB,IHC,ICC/IF
基因名称
ACLY
克隆号
OTI3G8
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.
计算分子量
121 kDa
实际分子量
120.8 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
Uniprot ID
P53396  
文献引用格式
ACLY Antibody (Clone#OTI3G8) (Boster Biological Technology, Wuhan, China. Catalog#M02372-2)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
ATP citrate lyase
蛋白名全称
ATP-citrate synthase
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100

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    Anti-ACLY Antibody (Clone#OTI3G8)

    筛选器: All WB IHC ICC/IF

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