Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:50 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Figure 1. Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ACLY monoclonal antibody (1:200).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACLY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACLY(Cat# M02372-2).
Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 4. Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 5. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 8. Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 9. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 10. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 12. Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 13. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 14. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 15. Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 16. Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 17. Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 18. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 19. Anti-ACLY mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY .
Figure 1. Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ACLY monoclonal antibody (1:200).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACLY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACLY(Cat# M02372-2).
Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 4. Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 5. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 8. Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 9. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 10. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 12. Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 13. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 14. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 15. Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 16. Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 17. Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 18. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)
Figure 19. Anti-ACLY mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY .