Figure 1. Western blot analysis using anti- ACLY antibody (PB10024). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: MOLT4 whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: U251 whole cell lysates,
Lane 5: 293T whole cell lysates,
Lane 6: HELA whole cell lysates,
Lane 7: T47D whole cell lysates,
Lane 8: HEPG2 whole cell lysates,
Lane 9: rat pancreas tissue lysates,
Lane 10: NIH/3T3 whole cell lysates.
Use rabbit anti- ACLY 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACLY at approximately 125KD. The expected band size for ACLY is at 121KD.
Figure 2. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 3. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 4. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 5. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 6. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 7. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 8. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 9. ICC analysis using anti- ACLY antibody (PB10024). was detected in immersion fixed A549 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
all(10) 
Figure 10. Flow cytometry analysis of HEPG2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(10) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis using anti- ACLY antibody (PB10024). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: MOLT4 whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: U251 whole cell lysates,
Lane 5: 293T whole cell lysates,
Lane 6: HELA whole cell lysates,
Lane 7: T47D whole cell lysates,
Lane 8: HEPG2 whole cell lysates,
Lane 9: rat pancreas tissue lysates,
Lane 10: NIH/3T3 whole cell lysates.
Use rabbit anti- ACLY 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACLY at approximately 125KD. The expected band size for ACLY is at 121KD.
Figure 2. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 7. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 8. IHC analysis using anti- ACLY antibody (PB10024). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 9. ICC analysis using anti- ACLY antibody (PB10024). was detected in immersion fixed A549 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 10. Flow cytometry analysis of HEPG2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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