Anti-ACLY Antibody (Clone#5I2)

Mouse monoclonal antibody

说明书

筛选器： All WB IHC FCM ICC/IF

M02372-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-ATP citrate lyase antibody (M02372-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Human placenta tissue lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: Caco-2 whole cell lysates,
Lane 5: HL-60 whole cell lysates,
Lane 6: Raji whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates,Use mouse Anti-ATP citrate lyase 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 121KD.
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Figure 2. Western blot analysis of anti-ATP citrate lyase antibody (M02372-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Rat testicular tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat brain tissue lysates,
Lane 5: Mouse lung tissue lysates,
Lane 6: Mouse testicular tissue lysates,
Lane 7: Mouse kidney tissue lysates,Use mouse Anti-ATP citrate lyase 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 121KD.
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Figure 3. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 4. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 5. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 6. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 7. Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
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Figure 8. ICC analysis using anti-ATP citrate lyase antibody (M02372-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ACLY Antibody (Clone#5I2)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ACLY

克隆号

5I2

抗体来源

Mouse

抗体亚型

IgG2b

免疫原

E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

计算分子量

121 kDa

实际分子量

127 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of°Cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and°Cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Uniprot ID

P53396

RRID

AB_3081866

文献引用格式

ACLY Antibody (Clone#5I2) (Boster Biological Technology, Wuhan, China. Catalog#M02372-1)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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实验步骤

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