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Anti-SOD2 Antibody (Clone#IDO-19)

Rabbit monoclonal antibody

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筛选器: All WB IHC

BM4813

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-SOD2 antibody (BM4813). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue lysates,
    Lane 2: rat small intestine tissue lysates,
    Lane 3: mouse brain tissue lysates,
    Lane 4: mouse small intestine tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOD2 antigen affinity purified monoclonal antibody (BM4813) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD2 at approximately 25 kDa. The expected band size for SOD2 is at 25 kDa.

    all(3)
  • IHC analysis of SOD2 using anti-SOD2 antibody (BM4813) .
    SOD2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOD2 Antibody (BM4813) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)
  • IHC analysis of SOD2 using anti-SOD2 antibody (BM4813) .
    SOD2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOD2 Antibody (BM4813) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品简介>

产品名称
Anti-SOD2 Antibody (Clone#IDO-19)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
IPO B; Mn SOD; MNSOD; SOD2; SODM
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
SOD2
克隆号
IDO-19
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human SOD2
计算分子量
25 kDa
实际分子量
25 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
Uniprot ID
P04179  
文献引用格式
SOD2 Antibody (Clone#IDO-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4813)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:1000-5000
Immunohistochemistry (IHC):1:50-200

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    Anti-SOD2 Antibody (Clone#IDO-19)

    筛选器: All WB IHC

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