Western blot analysis of anti-SOD2 (acetyl K68) antibody (M00349-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOD2 (acetyl K68) antigen affinity purified monoclonal antibody (M00349-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD2 (acetyl K68) at approximately 25 kDa. The expected band size for SOD2 (acetyl K68) is at 25 kDa.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
Western blot analysis of anti-SOD2 (acetyl K68) antibody (M00349-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOD2 (acetyl K68) antigen affinity purified monoclonal antibody (M00349-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD2 (acetyl K68) at approximately 25 kDa. The expected band size for SOD2 (acetyl K68) is at 25 kDa.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of anti-SOD2 (acetyl K68) antibody (M00349-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOD2 (acetyl K68) antigen affinity purified monoclonal antibody (M00349-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD2 (acetyl K68) at approximately 25 kDa. The expected band size for SOD2 (acetyl K68) is at 25 kDa.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2) .
SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOD2 (acetyl K68) Antibody (M00349-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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