Anti-SOD2 Antibody (Clone#2B12B1)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00349-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SOD2 using anti-SOD2 antibody (M00349-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HEPG2 whole cell lysates,
Lane 2: HCCP tissue lysates,
Lane 3: HCCP tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-SOD2 antigen affinity purified monoclonal antibody (M00349-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD2 at approximately 25 kDa. The expected band size for SOD2 is at 25 kDa.
all(4)
IHC analysis of SOD2 using anti-SOD2 antibody (M00349-3).
SOD2 was detected in a paraffin-embedded section of human Adenocarcinoma of the right colon tissue. The tissue section was incubated with mouse anti-SOD2 Antibody (M00349-3) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of SOD2 using anti-SOD2 antibody (M00349-3).
SOD2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with mouse anti-SOD2 Antibody (M00349-3) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of SOD2 using anti-SOD2 antibody (M00349-3).
SOD2 was detected in a paraffin-embedded section of human lymphoma tissue. The tissue section was incubated with mouse anti-SOD2 Antibody (M00349-3) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-SOD2 Antibody (Clone#2B12B1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

IPO B; Mn SOD; MNSOD; SOD2; SODM

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

SOD2

克隆号

2B12B1

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human SOD2, different from the related mouse sequence by one amino acid, and from the related rat sequence by four amino acids.

计算分子量

25 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SOD2(Superoxide Dismutase 2), also called IPO-B or MNSOD, is a mitochondrial matrix enzyme that scavenges oxygen radicals produced by the extensive oxidation-reduction and electron transport reactions occurring in mitochondria. This gene is a member of the iron/manganese superoxide dismutase family. Using a somatic cell hybrid panel containing different segments of chromosome 6, they demonstrated that SOD2 is located in the region 6q25.3-qter which, together with the FISH analysis, indicated that SOD2 is in the distal portion of 6q25. The SOD2 gene encodes an intramitochondrial free radical scavenging enzyme that is the first line of defense against superoxide produced as a byproduct of oxidative phosphorylation. Adeno-associated viral delivery of the human SOD2 gene resulted in suppression of optic nerve degeneration and rescue of retinal ganglion cells. The findings suggested that reactive oxygen species contributed to retinal cell death and optic nerve damage in mice with complex I deficiency, and that expression of SOD2 attenuated the disease process.

Uniprot ID

P04179

文献引用格式

SOD2 Antibody (Clone#2B12B1) (Boster Biological Technology, Wuhan, China. Catalog#M00349-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

superoxide dismutase 2, mitochondrial

蛋白名全称

Superoxide dismutase [Mn], mitochondrial

实验方案>

实验步骤

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