Western blot (WB): | 1:500~2000 |
Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT) and SOD2-Knockout HEK293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-SOD2 monoclonal antibody M00349-4 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOD2 monoclonal antibody.
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD2 (Cat# M00349-4).
Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT) and SOD2-Knockout HEK293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-SOD2 monoclonal antibody M00349-4 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOD2 monoclonal antibody.
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD2 (Cat# M00349-4).