Human c-Kit/CD117/KIT ELISA Kit

说明书

EK0835

96T ￥2480
货期：现货

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Human KIT/SCFR ELISA Kit standard curve
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产品简介>

产品名称

Human c-Kit/CD117/KIT ELISA Kit

规格/价格

96T/2480

指标别名

C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit

免疫原

sf21, Q26-T520

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10 pg/ml

检测范围

156 pg/ml - 10000 pg/ml

基因名称

KIT

基因名全称

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Uniprot ID

P10721

文献引用格式

Human c-Kit/CD117/KIT ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0835)

背景资料

SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0835-CAP	96-well plate precoated with anti- human KIT antibody	1
EK0835-ST	Lyophilized recombinant human KIT standard	50ng/tube×2
EK0835-DA	Biotinylated anti- human KIT antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	339	1218	4619	346	1138	4342
Standard deviation	23.39	64.55	314.09	26.98	60.31	329.99
CV(%)	6.9	5.3	6.8	7.8	5.3	7.6

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.037	0.195	0.347	0.635	0.871	1.373	1.713	2.092

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human c-Kit/CD117/KIT ELISA Kit

EK0835

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

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EK1581

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Human c-Kit/CD117/KIT ELISA Kit

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