Mouse c-Kit/CD117/KIT ELISA Kit

96T/2480

C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit

Expression system for standard: NS0; Immunogen sequence: S25-P527

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

156pg/ml-10000pg/ml

Kit

Mast/stem cell growth factor receptor Kit

P05532

Mouse c-Kit/CD117/KIT ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1581)

SCFR (Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Catalog number	Description	Quantity
EK1581-CAP	96-well plate precoated with anti- mouse CD117 antibody	1
EK1581-ST	Lyophilized recombinant mouse CD117 standard	10ng/tube×2
EK1581-DA	Biotinylated anti- mouse CD117 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	236	1951	5607	256	1869	5510
Standard deviation	15.81	85.84	409.31	20.48	89.71	495.9
CV(%)	6.7%	4.4%	7.3%	8%	4.8%	9%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.020	0.074	0.156	0.273	0.583	1.070	1.856	2.625

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h