产品名称
Human c-Kit/CD117/KIT Quick ELISA Kit
指标别名
C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit
免疫原
Expression system for standard: NS0; Immunogen sequence: Q26-T520
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
文献引用格式
Human c-Kit/CD117/KIT Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0835)
背景资料
SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
试剂盒内容
Catalog number | Description | Quantity |
FEK0663-CAP | 96-well plate precoated with anti-Human KIT antibody | 1 |
FEK0663-ST | lyophilized recombinant Human KIT standard | 10ng/tube×2 |
FEK0663-A | Human KIT Antibody A | 3ml |
FEK0835-B | Human KIT Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 342 | 1263 | 4698 | 351 | 1236 | 4352 |
Standard deviation | 20.18 | 54.31 | 272.48 | 24.92 | 69.22 | 278.53 |
CV (%) | 5.9% | 4.3% | 5.8% | 7.1% | 5.6% | 6.4% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
O.D. | 0.078 | 0.277 | 0.441 | 0.750 | 1.230 | 1.889 | 2.245 | 2.468 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)