Mouse c-Kit/CD117/KIT Quick ELISA Kit

96T/3580

C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit

Expression system for standard: NS0; Immunogen sequence: S25-P527

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

156pg/ml-10000pg/ml

Kit

kit oncogene

P05532

Mouse c-Kit/CD117/KIT Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1581)

SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

15分钟

Catalog number	Description	Quantity
FEK0663-CAP	96-well plate precoated with anti-Mouse CD117 antibody	1
FEK0663-ST	lyophilized recombinant Mouse CD117 standard	10ng/tube×2
FEK0663-A	Mouse CD117 Antibody A	3ml
FEK1581-B	Mouse CD117 Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	242	1796	5641	251	1651	5788
Standard deviation	13.55	88	389.23	17.32	84.2	324.13
CV (%)	5.6%	4.9%	6.9%	6.9%	5.1%	5.6%

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.060	0.159	0.229	0.373	0.636	1.111	1.806	2.395

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)