产品名称
Human Clusterin/CLU ELISA Kit
指标别名
AAG4; Apo J; APOJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; CLU; clusterin; Complement cytolysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; TRPM 2; TRPM2
免疫原
NSO, D23-R227(beta)&S228-E449(alpha)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78 ng/ml - 50 ng/ml
文献引用格式
Human Clusterin/CLU ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0914)
背景资料
Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0914-CAP | 96-well plate precoated with anti- human Clusterin antibody | 1 |
EK0914-ST | Lyophilized recombinant human Clusterin standard | 50ng/tube×2 |
EK0914-DA | Biotinylated anti- human Clusterin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1982 | 3789 | 21921 | 2148 | 4124 | 21708 |
Standard deviation | 120.9 | 219.76 | 920.68 | 135.32 | 259.81 | 1085.4 |
CV(%) | 6.1 | 5.8 | 4.2 | 6.3 | 6.3 | 5 |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.010 | 0.061 | 0.107 | 0.200 | 0.442 | 0.830 | 1.674 | 2.686 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Urine