产品名称
Human Clusterin/CLU Quick ELISA Kit
指标别名
AAG4; Apo J; APOJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; CLU; clusterin; Complement cytolysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; TRPM 2; TRPM2
免疫原
Expression system for standard: NS0; Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Clusterin/CLU Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0914)
背景资料
Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
试剂盒内容
Catalog number | Description | Quantity |
FEK0914-CAP | 96-well plate precoated with anti-Human CLU antibody | 1 |
FEK0914-ST | lyophilized recombinant Human CLU standard | 10ng/tube×2 |
FEK0914-DA | biotinylated anti-Human CLU antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1982 | 3789 | 21921 | 2148 | 4124 | 21708 |
Standard deviation | 120.9 | 219.76 | 920.68 | 135.32 | 259.81 | 1085.4 |
CV (%) | 6.1% | 5.8% | 4.2% | 6.3%% | 6.3% | 5% |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.013 | 0.070 | 0.127 | 0.236 | 0.469 | 0.913 | 1.819 | 2.780 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Urine