产品名称
Rat Clusterin/CLU ELISA Kit
指标别名
AAG4; Apo J; APOJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; CLU; clusterin; Complement cytolysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; TRPM 2; TRPM2
免疫原
Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat Clusterin/CLU ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1615)
背景资料
Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
试剂盒内容
Catalog number | Description | Quantity |
EK1615-CAP | 96-well plate precoated with anti- rat Clusterin antibody | 1 |
EK1615-ST | Lyophilized recombinant rat Clusterin standard | 10ng/tube×2 |
EK1615-DA | Biotinylated anti- rat Clusterin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 428 | 2367 | 10471 | 423 | 2436 | 10928 |
Standard deviation | 19.26 | 120.71 | 827.2 | 21.57 | 160.77 | 1060.01 |
CV(%) | 4.5% | 5.1% | 7.9% | 5.1% | 6.6% | 9.7% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.007 | 0.082 | 0.147 | 0.264 | 0.521 | 0.984 | 1.692 | 2.555 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)