产品名称
Mouse Clusterin/CLU ELISA Kit
指标别名
AAG4; Apo J; APOJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; CLU; clusterin; Complement cytolysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; TRPM 2; TRPM2
免疫原
Expression system for standard: NS0; Immunogen sequence: D22-R226 (beta) & S227-E448 (alpha)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Clusterin/CLU ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0923)
背景资料
Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0923-CAP | 96-well plate precoated with anti- mouse Clusterin antibody | 1 |
EK0923-ST | Lyophilized recombinant mouse Clusterin standard | 20ng/tube×2 |
EK0923-DA | Biotinylated anti- mouse Clusterin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 519 | 2684 | 8267 | 522 | 2616 | 7616 |
Standard deviation | 30.1 | 112.72 | 537.35 | 36.54 | 122.95 | 632.12 |
CV(%) | 5.8% | 4.2% | 6.5% | 7% | 4.7% | 8.3% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.024 | 0.108 | 0.163 | 0.286 | 0.516 | 1.002 | 1.754 | 2.437 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)