Anti-ACLY Antibody (Clone#OTI3G8)

Mouse monoclonal antibody

筛选器： All WB IHC ICC/IF ELISA

M02372-6

100μl ￥1960 150μl ￥2780
货期： 5-7天

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Figure 1. Western blot analysis of anti-LC3B/MAP1LC3B antibody (PA01524). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LC3B/MAP1LC3B antigen affinity purified polyclonal antibody (PA01524) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LC3B/MAP1LC3B at approximately 15,18 kDa. The expected band size for LC3B/MAP1LC3B is at 15 kDa.
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Figure 1. Western blot analysis of anti-LC3B/MAP1LC3B antibody (PA01524). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LC3B/MAP1LC3B antigen affinity purified polyclonal antibody (PA01524) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LC3B/MAP1LC3B at approximately 15,18 kDa. The expected band size for LC3B/MAP1LC3B is at 15 kDa.
all(7)
Figure 2. IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (PA01524).
LC3B/MAP1LC3B was detected in a paraffin-embedded section of human glioma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(7)
Figure 3. IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (PA01524).
LC3B/MAP1LC3B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(7)
Figure 4. IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (PA01524).
LC3B/MAP1LC3B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(7)
all(7)
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ACLY Antibody (Clone#OTI3G8)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, ELISA

基因名称

ACLY

克隆号

OTI3G8

抗体来源

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.

计算分子量

121 kDa

实际分子量

120.8 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Uniprot ID

P53396

RRID

ACLY Antibody (Clone#OTI3G8) (Boster Biological Technology, Wuhan, China. Catalog#M02372-2)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

ATP citrate lyase

蛋白名全称

ATP-citrate synthase

实验方案>

实验步骤

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