Anti-14-3-3 alpha/beta Antibody

筛选器： All WB IHC ICC/IF IP FCM

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  Figure 1. Western blot analysis of anti-14-3-3 alpha/beta antibody (BM4752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Caco-2 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat lung tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse lung tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 alpha/beta antigen affinity purified monoclonal antibody (BM4752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 alpha/beta at approximately 28 kDa. The expected band size for 14-3-3 alpha/beta is at 28 kDa.

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Anti-14-3-3 Antibody

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  Western blot analysis of 14-3-3 expression in Hela lysate.

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  Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 Antibody.

Anti-14-3-3 Epsilon/YWHAE Antibody

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate.

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Anti-14-3-3 Epsilon/YWHAE Antibody (Clone#3G11G2)

筛选器： All WB IHC FCM ICC/IF

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  Figure 1. Western blot analysis of anti- YWHAE Antibody (M01687-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: SH-SY5Y whole cell lysates,
  Lane 3: SW620 whole cell lysates,
  Lane 4: Raji whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: PC-12 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: NIH/3T3 whole cell lysates.
  Use mouse anti- YWHAE 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.

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  Figure 2. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 3. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 4. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 5. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of endometrial cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 6. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 7. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 8. IHC analysis using- YWHAE Antibody (M01687-2). detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 10. Flow cytometry analysis of ANA-1 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

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  Figure 11. Flow cytometry analysis of NRK cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

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  Figure 9. ICC analysis using anti- YWHAE Antibody (M01687-2). was detected in immersion fixed CACO-2 cell. Cells were stained using the Dylight594-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1141) and counterstained with DAPI (blue).

Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB FCM

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  Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.

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Anti-14-3-3 Sigma/SFN Antibody

筛选器： All WB IHC

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  Western blot analysis of 14-3-3 sigma expression in A431 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-14-3-3 Theta/YWHAQ Antibody

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  Western blot analysis of 14-3-3 Theta expression in HeLa cell lysate.

Anti-4EBP1/EIF4EBP1(Phospho-T70) Antibody

筛选器： All WB

Anti-5 Lipoxygenase/ALOX5 Antibody

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of 5 Lipoxygenase expression in K562 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-53BP2/TP53BP2 Antibody

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  Western blot analysis of ASPP2 expression in MCF7 cell lysate.

Anti-a-SMA/ACTA2 Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A549 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human 293T whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: human Caco-2 whole cell lysates,
  Lane 6: human SH-SY5Y whole cell lysates,
  Lane 7: human U251 whole cell lysates,
  Lane 8: human MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

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  Figure 2. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat heart tissue lysates,
  Lane 2: rat lung tissue lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: mouse heart tissue lysates,
  Lane 5: mouse lung tissue lysates,
  Lane 6: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

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  Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.

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  Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .

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Anti-a-SMA/ACTA2 Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM4172). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: rat C6 whole cell lysates,
  Lane 5: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM4172) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody.

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  Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

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Anti-a-SMA/ACTA2 Antibody (Clone#1A4)

筛选器： All WB IHC IF

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  Figure 1. Western blot analysis of anti-α-SMA antibody (BM0002). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: rat stomach tissue lysates,
  Lane 3: mouse stomach tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-α-SMA antigen affinity purified polyclonal antibody (BM0002) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for α-SMA at approximately 42 kDa. The expected band size for α-SMA is at 42 kDa.

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  Figure 2. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human endometrial carcinoma tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 6. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 7. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 8. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat heart muscle tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 9. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat lung tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with AEC (Catalog # AR1020) as the chromogen.

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  Figure 10. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 11. IF analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat lung tissue. Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 12. IF analysis of α-SMA using anti-α-SMA antibody (BM0002) and anti Anti-PECAM-1/CD31 antibody (PB9094).
  α-SMA was detected in a paraffin-embedded section of human placenta tissue. Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog#BA1126) and Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-A26C2/POTEG Antibody (Clone#OTI2B5)

筛选器： All WB

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  Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POTEG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POTEG (1:2000).

Anti-A2BP1/RBFOX1 Antibody (Clone#OTI1F2)

筛选器： All WB

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  Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBFOX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBFOX1.

Anti-A4GNT Antibody (Clone#OTI1F7)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A4GNT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-A4GNT.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-A4GNT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-A4GNT Antibody (Clone#OTI2H9)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A4GNT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-A4GNT.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-A4GNT mouse monoclonal antibody. (M13668) Dilution: 1:150

Anti-AAMP Antibody (Clone#29A85)

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  Western blot analysis of AAMP expression in (1) A375 lysate; (2) MCF7 cell lysate.

Anti-AANAT Antibody (Clone#OTI6E1)

筛选器： All WB

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  Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AANAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT (1:2000).