Anti-TNF Alpha/TNFA Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human U-937 whole cell lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: mouse spleen tissue lysates.
  Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.

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  Figure 2. IHC analysis using anti- TNFA/TNF Alpha antibody (BA0131). detected in paraffin-embedded section of human B lymphocytic tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.

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  Figure 3. Flow cytometry analysis of Caco-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB IHC FCM ELISA

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  Figure 2. IHC analysis using anti- YWHAG antibody (A04148-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 2. IHC analysis using anti- YWHAG antibody (A04148-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. Flow cytometry analysis of U87 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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Human ARMETL1/CDNF ELISA Kit

Anti-Beta Actin/ACTB Antibody

筛选器： All WB

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  Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human Jurkat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat kidney tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

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  Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: zebra fish tissue lysates,
  Lane 2: chicken heart tissue lysates,
  Lane 3: chicken liver tissue lysates,
  Lane 4: chicken brain tissue lysates,
  Lane 5: monkey heart tissue lysates,
  Lane 6: monkey lung tissue lysates,
  Lane 7: monkey kidney tissue lysates,
  Lane 8: monkey liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Anti-Beta Actin/ACTB Antibody

筛选器： All WB IHC ICC/IF

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  Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BM3873). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human GES-1 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human A431 whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: mouse PC-12 whole cell lysates,
  Lane 7: mouse Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified monoclonal antibody (BM3873) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

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Anti-Beta Actin/ACTB Antibody

筛选器： All WB IHC

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  Figure 1. Western blot analysis of anti- β-Actin antibody (BM0627).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat brain tissue lysates,Lane 6: Rat PC-12 whole cell lysates,Lane 7: Mouse brain tissue lysates,Lane 8: Mouse ANA-1 whole cell lysates,Lane 9: Rabbit intestines tissue lysates,Lane 10: Rabbit intestines tissue lysates,Lane 11: Pig intestines tissue lysates.Use rabbit anti- β-Actin 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.

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  Figure 2.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Mouse Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 3.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Rat Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 4.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Intestinal Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 5.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Lung Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 6.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Anti-GAPDH Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of anti- GAPDH antibody (A00227-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Caco-2 whole cell lysates,
  Lane 3: CCRF-CEM whole cell lysates,
  Lane 4: rat brain tissue lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: mouse brain tissue lysates,
  Lane 7: mouse liver tissue lysates.
  Use rabbit anti- GAPDH 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.

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  Figure 2. IHC analysis using anti- GAPDH antibody (A00227-1).detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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  Figure 3. IHC analysis using anti- GAPDH antibody (A00227-1).detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Anti-GAPDH Antibody

筛选器： All WB IHC ICC/IF

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  Figure 1. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human PC-3 whole cell lysates,
  Lane 6: monkey COS-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Figure 2. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat RH-35 whole cell lysates,
  Lane 4: mouse liver tissue lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.

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Anti-GAPDH Antibody (Clone#5A12)

筛选器： All WB ICC/IF IP

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  Figure 1. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human HepG2 whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: monkey COS-7 whole cell lysates,
  Lane 5: rat brain lysates,
  Lane 6: rat RH-35 whole cell lysates,
  Lane 7: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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七色多重荧光染色试剂盒Plus（六标七色）

三色多重荧光染色试剂盒Plus（双标三色）

五色多重荧光染色试剂盒Plus（四标五色）

六色多重荧光染色试剂盒Plus（五标六色）

四色多重荧光染色试剂盒Plus（三标四色）

荧光单标染色信号放大试剂盒（红光）

荧光单标染色信号放大试剂盒（绿光）

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  Figure 1. IF analysis of PCNA using anti-PCNA antibody (BM0104).
  PCNA was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PCNA Antibody (BM0104) at a dilution of 1:200. Fluorescent Single Stain Signal Amplification Kit (Green Light)(Catalog# PTSA-12G) was used to enhance the signal.

0.02%EDTA溶液

0.05%胰酶溶液(不含EDTA)

0.05%胰酶溶液(含EDTA)

0.25%胰酶溶液(不含EDTA, 含酚红)