Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- HMOX1 antibody (A00253-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat RH35 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
Use rabbit anti- HMOX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMOX1 at approximately 33KD. The expected band size for HMOX1 is at 33KD.
Figure 1. Western blot analysis of anti- HMOX1 antibody (A00253-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat RH35 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
Use rabbit anti- HMOX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMOX1 at approximately 33KD. The expected band size for HMOX1 is at 33KD.