Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of anti-HMOX1 antibody (PB9212). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: HELA Whole Cell Lysate.
Use rabbit anti-HMOX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMOX1 at approximately 33KD. The expected band size for HMOX1 is at 33KD.
Figure 2. IHC analysis of HMOX1 antibody (PB9212). was detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of anti-HMOX1 antibody (PB9212). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: HELA Whole Cell Lysate.
Use rabbit anti-HMOX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMOX1 at approximately 33KD. The expected band size for HMOX1 is at 33KD.
Figure 2. IHC analysis of HMOX1 antibody (PB9212). was detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.